FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17919603
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACCG27650.084.068131
TTACGCG10900.083.1330649
CGCCGAA27150.080.7701341
TTTGCGC27800.074.9375461
CGGTACG30650.074.6471561
TGCGCGA25400.074.248111
GCGCTAG31450.070.656541
ATAACGC35250.069.6754151
GTGCGAC31700.069.407541
GGCGACT37250.068.6817251
CGAACGC32750.066.5126651
CCGCTAT35550.066.208791
GACGCGT27600.065.946181
TAGGCGC35250.065.528071
CGCGGTA35400.065.0439151
TCGACGG31250.064.325341
AACGCGT27400.064.290213
GTTCGCG36450.063.442968
TTACCCG33300.063.4385151
GCGCGTA29750.063.1459581
Produced by FastQC (version 0.11.5)