FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17919603
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT25850.0100.67726
ACGACGC27950.093.372275
CACGACG30300.086.369774
GACGCTC39550.066.3520667
TACACGA42200.063.087622
CTACACG44050.060.1472241
ACGCTCT47100.055.5617878
ACACGAC49350.054.0577353
CGCTCTT58700.045.1994259
TCTTCCG83800.07.30110510-14
CTTCCGA90850.07.24521210-14
CGTAGAG28305.456968E-127.2414331
TTCCGAT109900.06.187216310-14
AACGGGG34359.276846E-116.17908571
CGCTCGG14200.00267598946.1293316145
CGATGAG26901.9012623E-75.98581551
CGAAAAG34506.6393113E-105.9400741
TCCGATC111050.05.80982215-19
CGTTGAG25302.4562469E-65.7857861
CCGATCT120200.05.644984215-19
Produced by FastQC (version 0.11.5)