FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485790

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485790
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18075539
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27650.0103.2901156
ACGACGC28050.0102.3345
CACGACG29900.096.0020454
GACGCTC40350.070.779977
TACACGA44650.064.860472
CTACACG45050.064.342841
ACGCTCT47250.060.137028
ACACGAC49050.058.979963
CGCTCTT58900.047.9962169
TCTTCCG79350.08.00267210-14
CTTCCGA84300.07.876725710-14
CGCTGAG36000.07.72646671
TTCCGAT102500.06.789303310-14
TCCGATC103650.06.50480515-19
CCGATCT114450.06.11902115-19
CGATCTA30900.05.912384515-19
TAACCCT478200.05.72980556
CTAACCC494400.05.67401035
CGTTGAG24659.708929E-65.64204451
CCGTAGT15600.00755040775.58322762
Produced by FastQC (version 0.11.5)