FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21274421
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA31000.082.192881
TTACGCG12150.081.142889
TTTGCGC34550.075.8667761
CGCCGAA34300.073.858181
ACGACCG31250.072.866351
GTGCGAC35150.072.072141
GCGCTAG37200.068.887711
TTACCCG40950.068.658451
CGGTACG30300.068.1432951
AACGCGT27400.066.996313
CGAACGC40400.066.6934361
GGCGACT42600.065.483521
GCGCGTA37850.065.189951
CCGATAA47250.064.772571
GTTCGCG39100.064.14868
AACCGCG37400.063.6250881
CCGCTAT41000.063.574481
ATAACGC39650.063.3384671
GTTACGC31750.062.7880178
GACGCGT34950.062.009921
Produced by FastQC (version 0.11.5)