FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19994673
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12550.085.488219
TGCGCGA29950.080.1576161
CGCCGAA30800.078.420751
TTTGCGC32900.076.752221
GCGCTAG33650.073.736481
GTGCGAC32700.072.297381
TCACGCG28250.069.027498
ACGACCG29300.068.446421
CGGTACG31300.068.0481641
GGCGACT38850.067.069761
TAGGCGC36900.065.655241
ATAACGC38350.065.6539461
CGAACGC38700.065.627561
GTTACGC32200.065.512688
GCGCGTA34100.064.607051
GTACCGT43000.063.8938378
TTCCGCG20100.063.8359687
CCGCTAT40500.063.614391
GTACGCT29100.063.025428
GTTCGCG41600.062.907458
Produced by FastQC (version 0.11.5)