FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18077003
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11100.080.9734659
GTGCGAC31500.079.89981
TGCGCGA27150.078.95791
CGCCGAA28700.078.772461
ACGACCG28450.078.693161
TTTGCGC27350.078.113011
ATAACGC30750.074.2347641
CGGTACG29150.072.285591
GCGCTAG30000.070.23751
CCGCTAT33400.068.344881
GCGCGTA26550.065.8614351
GTACGCT25950.065.64098
GACGCGT28650.065.119851
GTACCGT39600.064.43058
TTACCCG33800.063.6397481
CGAACTA39000.063.5964551
GGCGACT35700.063.5318221
GCCGGAT36650.063.482051
TCGTACG38050.063.4356778
AACGCGT26000.062.7828943
Produced by FastQC (version 0.11.5)