FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19843241
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGAA30350.081.703381
TTACGCG10650.077.589179
TGCGCGA30300.076.285761
CGGTACG31250.075.137031
TTTGCGC31600.073.1474151
ACGACCG29850.071.064491
GCGCTAG33150.070.830531
GTGCGAC33650.070.647581
TTACCCG36950.070.2769551
CGAACGC36050.068.784961
ATAACGC36000.067.05181
GACGCGT29250.066.020231
TCGACGG34950.065.926851
GGCGACT39550.065.287081
CGTTGCG14350.064.655277
CGATTCG24350.064.635574
TAGGCGC37800.064.439391
CCGCTAT38200.064.339091
AACGCGT27600.063.8746343
GTACCGT46150.062.9823078
Produced by FastQC (version 0.11.5)