FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19756027
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12250.084.031739
TGCGCGA29700.082.5764851
ACGACCG28750.078.939051
TTTGCGC30700.077.978961
GTGCGAC36550.075.913651
CGCCGAA30650.075.4787451
CGGTACG29850.072.35121
GCGCTAG35050.069.7632141
ATAACGC37700.069.714181
CGAACGC35700.069.108221
GGCGACT39400.066.52041
TTACCCG37200.065.9281
GCGCGTA31800.065.151941
GTTACGC29650.064.790648
CGATCGG37950.064.625081
CCGCTAT36500.064.58491
GACGCGT30350.064.405151
TAGGCGC36700.063.0360571
GCCGGAT36450.061.2590641
GTCGGAC30300.061.1288071
Produced by FastQC (version 0.11.5)