FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485812

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485812
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20294309
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11800.090.309619
ACGACCG34000.081.174021
TGCGCGA33050.080.184721
TTTGCGC34150.079.9599841
CGCCGAA30850.076.41091
CGGTACG33100.074.534351
GTGCGAC34150.070.956441
GACGCGT31700.067.895751
GGCGACT41100.067.863731
GCGCTAG32200.065.932071
ATAACGC36550.065.4960251
CGAACGC34700.064.768441
TCGCGAT26100.064.438618
TTACCCG38350.064.3308261
GCGCGTA31300.063.8517571
CCGCTAT39500.063.7552451
CTTACCG41800.063.7499241
AACGCGT30300.063.4005973
CCGATAA41350.063.3814351
CGTTGCG15650.062.9974867
Produced by FastQC (version 0.11.5)