FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16962598
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT31800.0109.64166
ACGACGC33550.0105.002555
CACGACG34550.0101.33374
GACGCTC43450.079.743547
TACACGA47500.074.267632
CTACACG48900.072.614731
ACGCTCT50300.068.739728
ACACGAC54050.065.731613
CGCTCTT59850.058.0134549
TCTTCCG83450.09.17311810-14
CTTCCGA93550.08.50820510-14
TTCCGAT111750.07.135502310-14
TCCGATC109450.07.10063715-19
CCGATCT118350.06.87294615-19
CGATGAG27951.9463187E-106.8105691
CGATCTA32000.06.434083515-19
CGGTAAG16550.00209512515.75091841
ACCGGGT20156.4445205E-45.4501451
TAACCCT584250.05.32250366
CGATGGT36458.3400664E-85.222371
Produced by FastQC (version 0.11.5)