FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16783702
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27450.0104.051896
ACGACGC28900.099.332975
CACGACG29650.096.820334
GACGCTC39150.072.955267
TACACGA43500.066.374982
CTACACG43750.065.9204251
ACGCTCT45600.062.7945378
ACACGAC47000.061.2334483
CGCTCTT56850.050.750729
TCTTCCG78150.08.29278110-14
CTTCCGA83200.08.05082310-14
TTCCGAT99150.07.22363610-14
TCCGATC99450.06.793636315-19
CCGATCT110050.06.415936515-19
TCGCCGG15650.00120073586.024924145
TAACCCT563200.05.7278746
CTAACCC585050.05.66264445
CGATCTA27900.05.612297515-19
CGCTGAG34151.9477739E-85.5729421
CCTAACC607800.05.5222544
Produced by FastQC (version 0.11.5)