FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18957728
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12600.089.176599
TTTGCGC32550.082.651381
TGCGCGA28150.080.767251
CGCCGAA30150.077.591821
ACGACCG27150.072.4328841
TTACCCG36400.071.097641
GCGCTAG32900.070.661771
CGGTACG27800.070.476331
GTGCGAC34400.070.1308061
CGAACGC33950.068.907031
GCGCGTA31650.068.832871
GACGCGT29950.067.369851
GTTACGC30350.067.356598
ATAACGC36050.066.515351
CGAACTA40700.064.8434451
TCGTACG35400.064.710328
TAGGCGC34900.064.098711
CCTTACG34700.064.04681
TCGCGAT22850.063.767528
CCGCTAT36600.063.5183681
Produced by FastQC (version 0.11.5)