FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19007391
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12650.091.118439
TTTGCGC33950.079.863441
TGCGCGA28250.078.338371
ACGACCG29050.075.172021
GTGCGAC34350.072.9601061
TTACCCG38000.071.5445251
CGCCGAA29550.070.9242251
ATAACGC35700.069.995841
GCGCTAG31850.068.333411
CGGTACG27900.066.713791
GGCGACT37500.065.8543851
TCCGCGA31450.065.4630368
GCGCGTA32600.065.4126051
TCGTACG33950.065.340168
ACGCCGA35200.064.952821
GTTACGC29600.063.4317258
TCACGCG27050.062.7114838
TTCCGCG19300.062.3518377
ATTAGCG37350.061.9986951
TACGAAC41450.061.8770451
Produced by FastQC (version 0.11.5)