FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19264115
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA29150.081.7374341
GCGCTAG32650.079.711571
GTGCGAC35700.077.213971
TTACGCG12200.076.652259
CGGTACG30600.074.749691
GACGCGT30400.074.035671
TTTGCGC30450.073.91411
ACGACCG29250.072.936231
CGCCGAA29900.072.331431
CCGCTAT37500.068.815711
CGAACGC33500.067.403451
CGTTGCG14200.067.388577
TCCGCGA26100.066.383198
GCCGGAT39000.066.168951
TCGCGAT20650.065.648128
GGCGACT35750.065.62211
GCGCGTA31850.064.910641
TCACGCG23800.064.574298
TAGGCGC36200.064.401321
CAACGCG23650.064.370798
Produced by FastQC (version 0.11.5)