FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20686186
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11350.088.7801749
TTTGCGC34150.078.342561
TGCGCGA28150.077.074171
CGCCGAA31450.074.580441
ACGACCG29600.074.289111
GTGCGAC35050.073.6122741
CGGTACG32650.070.941361
ATAACGC40200.069.6518861
GCGCTAG32400.069.226431
CGATCGG39450.068.003251
TTACCCG40600.067.340821
GTTACGC29500.066.596548
TCACGCG31800.066.33928
GGCGACT36350.065.13191
CCGCTAT40800.065.034531
TAGGCGC36050.064.453961
GCCGGAT37650.064.245771
TTCCGCG22950.064.123587
GACGCGT33000.063.9696731
TCCGCGA30950.063.7107438
Produced by FastQC (version 0.11.5)