FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20509938
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12900.083.730959
TTTGCGC28700.078.562261
CGCCGAA33450.075.503691
TGCGCGA29650.074.06991
ACGACCG30050.072.840331
GTGCGAC32400.070.268481
TAGGCGC40600.069.419241
GCGCTAG36200.068.554561
CGGTACG30300.068.373691
GTTACGC30850.067.909758
GACGCGT31900.067.6981051
TTACCCG39550.067.56031
GCGCGTA29550.067.136241
CCGCTAT39400.066.516911
GCCGGAT38400.065.008231
CGAACGC37200.064.547011
GGCGACT41000.064.278221
TTCCGCG20150.064.0375067
ATAACGC36900.063.0878871
TCCGCGA29700.062.4845968
Produced by FastQC (version 0.11.5)