FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005485843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20509938
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGCGA1997736897.40335636314454No Hit
NATAGCGA1179010.5748481540997344No Hit
AATAGCGA458850.2237208128079178No Hit
CATAGGGA371510.18113657876488948No Hit
CAGAGCGA262280.12787946994281504No Hit
CATAGCAA250960.12236019436041201No Hit
CATAGAGA242360.11816710513703162ABI Solid3 Adapter B (100% over 8bp)
CATAGCGC235570.11485651492461849No Hit
CATAGCGG235340.11474437416631879No Hit
GATAGCGA221750.10811831805634907No Hit
TATAGCGA208140.10148251057609244No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)