FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20654668
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13600.080.4888159
TGCGCGA33250.079.3782351
GTGCGAC36100.076.564021
TTTGCGC35300.076.2222751
CGGTACG33050.075.8656541
ACGACCG29650.075.663661
CGCCGAA34450.074.2722851
GCGCTAG36200.072.504511
GACGCGT32500.070.83321
TCGCGAT26450.067.423478
CGAACGC38700.067.062991
GGCGACT42750.066.1975251
GCGCGTA34700.066.131051
AACCGCG38650.065.252851
CCGCTAT38950.064.938491
CCTTACG36800.064.747961
ATAACGC35900.064.328981
TCGTACG38900.063.7350278
TCACGCG27850.063.253248
CGCGGTA42150.062.7914051
Produced by FastQC (version 0.11.5)