FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20564404
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12100.086.871219
TTTGCGC34000.078.2765351
CGCCGAA32850.076.565331
TGCGCGA31300.074.283421
ACGACCG29750.072.2552641
CGGTACG33650.070.8339841
GTGCGAC36600.070.7182161
GCGCGTA36500.070.1106951
GCGCTAG34200.068.839681
GTTACGC30400.065.577128
AACCGCG39800.065.0323261
GGCGACT43650.064.991511
TCACGCG28050.064.868538
GACGCGT34350.063.856281
GTTACCG30200.063.851038
CTTACCG44300.063.7078131
CCGATAA42900.062.378211
CCGCTAT41600.062.218431
AACGCGT29300.061.6062743
CGAACGC37850.061.4285621
Produced by FastQC (version 0.11.5)