FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19843241
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTGAC1932745397.4006867124176No Hit
NACTTGAC1766170.8900612556184748No Hit
AACTTGGC365870.18438016249462474No Hit
ANCTTGAC309220.15583139871153104No Hit
AACGTGAC260590.13132431340223102TruSeq Adapter, Index 21 (100% over 8bp)
CACTTGAC243310.12261605853600226No Hit
AACTTGAA236150.11900777700578247No Hit
AACTTGCC226570.11417993663434314No Hit
AGCTTGAC198640.10010461496687964No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)