FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16921259
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC26650.081.7999651
TTACGCG9650.080.371699
TGCGCGA27200.078.2633061
ACGACCG25650.074.721921
CGGTACG29200.073.654381
CGCCGAA27000.072.882391
GTGCGAC30000.070.471041
GCGCTAG29400.068.4257351
GGCGACT32800.068.246711
ATAACGC31150.067.6345441
CGAACGC32050.066.876521
TTACCCG34500.066.792171
GACGCGT24750.065.911891
CCGCTAT31700.065.5379941
TCCCGAT38950.064.795631
GTACCGT39950.064.592168
ACGCCGA32750.064.106891
GCGCGTA27300.063.7746961
CGATTCG20800.063.112224
GCCGGAT30900.062.9734151
Produced by FastQC (version 0.11.5)