FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16823617
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG9900.088.602329
TGCGCGA27300.080.712391
TTTGCGC27950.080.144911
ACGACCG24600.076.775191
CGGTACG25050.074.811531
GCGCGTA25700.074.628461
GTGCGAC29250.074.580741
CGCCGAA28050.072.551811
GCGCTAG27850.072.284281
TTACCCG32750.069.292581
GACGCGT25250.067.550861
CCGCTAT32000.067.256411
CGAACGC30700.066.527651
GGCGACT33050.066.448651
ATAACGC32100.066.362731
CGAACTA33550.065.021961
TCGTTCG26900.064.4081048
CGAACGT18450.064.176173
AACCGCG32750.063.7044681
TCGCGAT18350.063.2089848
Produced by FastQC (version 0.11.5)