FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17983882
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11850.082.585829
TGCGCGA26050.079.8032761
CGCCGAA29700.079.3614351
CGGTACG29650.078.2608641
ACGACCG27500.077.458741
TTTGCGC30450.076.685551
GTGCGAC32550.073.312281
GCGCGTA25550.069.905121
GCGCTAG31400.069.003671
GCCGGAT35700.065.8183441
CCGCTAT33500.065.552091
GACGCGT27600.065.243331
GGCGACT37200.064.5417941
TCCGCGA24950.064.50198
ATAACGC33000.064.3271261
TTACCCG35150.063.724471
GTACGCT24750.063.5586438
AACCGCG34050.063.203381
CGATCGG31350.061.4084781
TCGTACG36750.061.3469738
Produced by FastQC (version 0.11.5)