FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17983882
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29500.0102.9507756
ACGACGC30000.0101.71735
CACGACG31450.097.262154
GACGCTC42400.072.31397
TACACGA48200.065.423012
CTACACG49050.064.521481
ACGCTCT48450.063.2839858
ACACGAC51300.060.4755333
CGCTCTT61650.049.6165589
TCTTCCG82150.08.57785410-14
CTTCCGA88000.08.38658210-14
TTCCGAT101100.07.52936110-14
CGTTGAG25903.6379788E-117.32027151
CGATCTA30050.07.28527915-19
TCCGATC100500.07.054342315-19
CCGATCT111450.06.5693915-19
CGGTGAT37006.675691E-105.71544271
ATCGGGG22951.20691366E-45.4015681
CTAACCC502400.05.22267445
TAACCCT482450.05.15322886
Produced by FastQC (version 0.11.5)