FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19543271
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA27200.077.8523941
CGCCGAA32550.077.662461
GTGCGAC32650.074.9559861
TTTGCGC28650.074.167971
ACGACCG31250.073.8590241
TTACGCG10700.072.49289
CGGTACG29400.071.029791
TTACCCG35100.070.976541
GCGCGTA31350.070.117551
GCGCTAG33750.069.9077151
ATAACGC37600.067.816351
GTACCGT45700.066.306338
TCACGCG25050.065.692088
CCGCTAT38850.064.3141251
CGAACGC36150.064.253081
TCGCGAT21200.063.944128
TAGGCGC37100.063.3977931
GCCGGAT36450.062.518121
CGATCGG35400.062.5095941
ACGCCGA40450.061.9513321
Produced by FastQC (version 0.11.5)