FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21069070
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA33000.083.635051
TTACGCG12650.080.228019
GTGCGAC36300.075.628511
ACGACCG34200.075.1349261
GACGCGT34800.073.629131
CGCCGAA32900.072.985881
TTTGCGC32600.072.3101351
GCGCGTA37050.071.331261
CGGTACG32350.070.379641
ATAACGC37650.069.611171
GCGCTAG38100.068.981131
ACGCCGA40350.067.856111
GGCGACT40250.065.66021
AACCGCG41700.065.132651
CCGCTAT42050.064.416421
TAGGCGC38700.062.4257621
TCGTACG41550.062.285328
CTTACCG46400.061.375141
TAAACCG47700.061.2372131
GTACCGT50600.061.1738628
Produced by FastQC (version 0.11.5)