FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485922

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485922
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20683170
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA31750.081.606941
TTACGCG12900.079.2354359
ACGACCG30750.076.644031
CGCCGAA31700.074.1162341
TTTGCGC33200.072.751741
CGGTACG35200.071.113331
GGCGACT43850.070.271641
GCGCTAG35300.069.460461
GTGCGAC34700.068.974071
AACCGCG37350.067.215761
CTTACCG46750.066.3822251
TCACGCG28050.066.160418
CGAACGC39850.065.754011
AACGCGT29350.065.74823
ATAACGC36700.065.6141361
GACGCGT33600.064.697091
CGTTGCG15950.063.6294867
GTTCGCG45750.063.5395858
CCGCTAT38650.063.4399531
CGCGGTA40500.063.4336431
Produced by FastQC (version 0.11.5)