FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19276363
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCGC32150.083.334541
TTACGCG11500.081.3175359
TGCGCGA27850.078.327821
ACGACCG27800.078.2053761
CGGTACG29850.074.5511251
GTGCGAC32950.074.202071
CGCCGAA32400.070.265211
GACGCGT31100.069.6716841
AACGCGT27600.068.424463
CGAACGC35300.068.225411
CCGCTAT36500.066.985151
TTACCCG38300.066.895091
ATAACGC36500.064.9796141
GCGCTAG32100.064.764691
GTTACGC28650.064.522018
TAGGCGC37100.064.323351
GCCGGAT38700.064.312131
GTTCGCG39800.064.113868
GCGCGTA28850.063.1798361
TCCGCGA28150.063.0928348
Produced by FastQC (version 0.11.5)