FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485928

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485928
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19103562
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11550.082.84739
ACGACCG29250.078.5807951
TTTGCGC30900.078.1750261
CGCCGAA30050.075.027231
GTGCGAC32450.073.9897841
CGGTACG28850.073.834581
TGCGCGA29450.072.578861
GCGCTAG32500.070.047011
ATAACGC35450.069.999681
GGCGACT37600.067.9438551
GACGCGT28900.067.374631
CGAACGC35700.066.0237661
GCCGGAT36850.065.751121
TAGGCGC34850.065.113581
CCGCTAT33650.064.172591
AACGCGT27150.064.133833
CGAACTA43050.063.763291
GCGCGTA31550.063.3396871
TCACGCG27150.063.2799388
GTTACGC29800.063.004428
Produced by FastQC (version 0.11.5)