FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19103562
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT31600.0102.77576
ACGACGC33000.099.084685
CACGACG34150.096.597214
GACGCTC44200.073.4776467
TACACGA49350.068.030142
ACGCTCT50200.065.27268
CTACACG53500.062.8181531
ACACGAC55950.059.7376443
CGCTCTT63050.051.1647729
CTTCCGA95600.08.40197910-14
TCTTCCG93250.08.17836810-14
TTCCGAT115900.06.967893610-14
TCCGATC114600.06.55364415-19
CGCTGAG37150.06.47579531
CCGATCT127250.06.255359615-19
CGATCTA33900.05.902243615-19
CGTTGAG26258.196639E-75.8321411
TAACCCT787200.05.7188156
CTAACCC831700.05.6051615
ACCCTAA855250.05.5279242
Produced by FastQC (version 0.11.5)