FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485937

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485937
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20206375
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13500.086.45339
TGCGCGA32500.083.795411
CGCCGAA32300.078.647991
GCGCTAG35300.074.660091
TTTGCGC33650.072.882021
ATAACGC39250.069.7577741
GTGCGAC34850.069.322121
ACGACCG28000.069.286481
TAGGCGC36300.069.1748351
CGGTACG32600.067.594221
GGCGACT40150.067.464741
TCACGCG28650.067.051858
CGAACGC39400.066.5192641
GTTACGC32650.066.386158
GCGCGTA34800.066.055821
GCCGGAT38450.065.8780061
CCGCTAT38900.065.8687061
GACGCGT32550.064.549311
TTACCCG37350.064.290081
TTCCGCG20300.064.278537
Produced by FastQC (version 0.11.5)