FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21467511
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA33250.079.077551
ACGACCG30650.076.2273251
TTACGCG15350.074.617179
CGCCGAA33900.073.2403641
GCGCTAG37500.072.2638241
TTTGCGC32950.071.128721
TCGCGAT25050.068.295858
GTGCGAC35250.068.1498951
GCGCGTA36650.067.744841
CGAACGC40300.067.2430041
GGCGACT43550.066.765611
CGGTACG31200.066.66751
GTTCGCG43650.065.267718
ATAACGC41500.064.5927051
AACCGCG40400.064.5385361
AACGCGT30650.064.2336353
CCTTACG41450.064.140531
TCGTACG40050.063.8943188
TTACCCG39250.063.257281
GCCGGAT45000.062.6611981
Produced by FastQC (version 0.11.5)