FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19552019
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG11450.080.4058469
CGGTACG29450.078.286751
TGCGCGA30850.076.632041
CGCCGAA30900.075.323711
GTGCGAC34500.074.2526551
ACGACCG26700.072.369521
TTTGCGC31500.072.030131
CGAACGC34150.070.941491
GACGCGT31900.068.6030651
TCACGCG27250.067.570418
TTACCCG36950.067.546521
GGCGACT38100.065.507711
GCGCGTA30850.065.2439961
GCGCTAG32400.065.059471
GTTACGC27400.064.554818
GTACCGT44100.064.272848
ACGCCGA37850.064.200031
ATAACGC35100.064.016851
GTACGCT29000.061.9930538
TAGGCGC35900.061.7747841
Produced by FastQC (version 0.11.5)