FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20708016
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG14100.084.317199
CGCCGAA32250.077.176711
TTTGCGC32600.077.021781
CGGTACG32400.076.367531
GTGCGAC35350.071.858331
TGCGCGA31300.071.3333361
ACGACCG29350.070.336061
TTACCCG39550.069.039771
ATAACGC40500.068.685591
GCGCTAG34150.068.166891
GTTACGC32950.067.762088
GACGCGT33650.067.439391
CCTTACG38150.067.159971
CCGCTAT41350.066.919591
CGTTGCG16750.065.35127
TAGGCGC38500.065.0283051
GCCGGAT39750.064.088361
GCGCGTA34200.064.000311
AACCGCG39200.063.867081
AACGCGT31850.063.6347273
Produced by FastQC (version 0.11.5)