FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17790437
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26000.081.4519961
TTACGCG11950.080.682489
TTTGCGC29050.075.674991
CGGTACG26500.073.555141
CGCCGAA27800.073.5422061
ACGACCG25900.073.278611
ATAACGC33800.070.02661
GCGCTAG28850.069.5956341
CCGCTAT31500.069.323891
CGAACGC34900.067.399531
GACGCGT27900.066.187131
GGCGACT35250.065.898781
AACCGCG33750.065.787921
GTTCGCG36350.065.21378
TCGACGG33200.064.449871
GCGCGTA27700.064.0195851
GTGCGAC32050.063.7901151
AACGCGT24150.063.656993
GTTACGC26450.063.3113828
ACGCCGA34850.062.2395061
Produced by FastQC (version 0.11.5)