FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17790437
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT26200.098.220626
ACGACGC27350.095.150865
CACGACG29350.088.913984
GACGCTC37500.068.816777
TACACGA44500.059.5883752
ACGCTCT43400.058.965068
CTACACG44450.058.951591
ACACGAC46700.055.880623
CGCTCTT59350.043.484969
AACGGGG32301.8189894E-126.8365351
TCTTCCG84950.06.758846310-14
CTTCCGA89000.06.695647710-14
CGTTGAG23951.2264536E-76.4540311
CGATCTA27900.06.0798415-19
CGATGGG30003.8198777E-85.8885351
TCCGATC112350.05.81987915-19
GTTCCGA35451.6261765E-95.7255525
TTCCGAT111200.05.71097310-14
CCGATCT122050.05.440492615-19
CGATGGA30401.8044138E-65.32681
Produced by FastQC (version 0.11.5)