FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17985820
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12350.084.5233849
TGCGCGA27050.081.4846341
ACGACCG26300.079.3385541
CGGTACG29050.078.4038241
CGCCGAA27950.074.129141
GCGCTAG30000.072.002531
GTGCGAC30850.071.685791
TTTGCGC28000.071.372771
ATAACGC36650.068.760951
GCGCGTA26000.067.5377041
TCGTACG34800.065.824748
AACGCGT30650.065.74873
TTACCCG32100.065.2321241
GCCGGAT36850.065.1976551
GACGCGT27300.064.321621
GGCGACT37450.063.7607231
GTTCGCG38350.063.7009438
TGACGGC39400.063.2157361
TAGGCGC35900.062.4204751
ATTAGCG38650.062.3513761
Produced by FastQC (version 0.11.5)