FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19963133
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACTTGAC1907722195.56225969140215No Hit
NACTTGAC4151282.079473196917538No Hit
CACTTGAC419330.2100521997223582No Hit
AACTTGCC344010.17232265095864463No Hit
ANCTTGAC319730.16016023136248203No Hit
AACTTGGC290690.14561341649128923No Hit
TACTTGAC266230.13336083068724733No Hit
AGCTTGAC259740.13010983796982167No Hit
GACTTGAC249340.12490023484790688No Hit
AACTTGAA238980.1197106686610764No Hit
ACCTTGAC229800.11511219205923238No Hit
AACTTGAG206250.10331544652835804No Hit
AACTTGTC201770.10107130979891783No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)