FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18757712
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG13050.088.8722469
TTTGCGC29700.078.9977341
TGCGCGA27600.078.552091
CGCCGAA30050.075.112651
GTGCGAC33800.074.68721
TTACCCG35400.073.6186451
ACGACCG26850.072.726961
ATAACGC34600.071.243611
GCGCGTA31100.070.428041
TCCGCGA28300.068.6443948
TCGTACG37200.067.615038
GTTACGC32700.067.387998
GACGCGT30250.066.270811
CGTTGCG16500.065.896757
CGAACTA38550.065.67711
CGAACGC36950.065.305971
GCGCTAG32200.064.793941
CGGTACG31400.063.8437041
GGCGACT36550.063.786131
TCACGCG26900.063.5940368
Produced by FastQC (version 0.11.5)