FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19119415
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10200.081.729919
CGCCGAA31850.078.2733151
ACGACCG29200.076.562161
TTTGCGC31700.074.235931
TGCGCGA29650.074.160041
GTGCGAC32700.073.989761
CGGTACG29100.073.539971
GCGCTAG31750.072.9609151
TTACCCG38750.069.459691
CGTTGCG13200.068.647567
TAGGCGC35450.067.420291
ATAACGC35850.066.873171
ACGCCGA35200.066.8545151
CGCGGTA36550.066.397241
GCCGGAT38000.065.411871
GACGCGT30650.065.262231
AACGCGT28200.063.2375533
GTACCGT41750.063.0279968
CCGCTAT38050.063.006651
ACTACGA41400.062.8820041
Produced by FastQC (version 0.11.5)