FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19119415
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT29950.0101.889336
ACGACGC30200.0101.046145
CACGACG32200.094.994844
GACGCTC44100.069.361317
TACACGA46800.066.2962652
CTACACG51150.062.5669441
ACGCTCT52400.058.37538
ACACGAC53550.057.5271533
CGCTCTT65850.046.4520269
CTTCCGA85300.08.54868710-14
TCTTCCG84700.08.24981310-14
TTCCGAT97900.07.62614410-14
TCCGATC96100.07.482370415-19
CGCTAAG16555.1826064E-67.1289051
CCGATCT105050.06.969093315-19
CGATCTA29750.06.627242615-19
CGTTGAG25751.0084477E-86.58645061
ACGGGAG40100.06.43612581
CGCTGAG38551.8189894E-126.31234031
TAACCCT762000.05.8405926
Produced by FastQC (version 0.11.5)