FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16605348
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT27000.0103.6284566
ACGACGC29100.097.146195
CACGACG29200.096.564394
GACGCTC37300.075.013017
CTACACG43500.067.293251
TACACGA43900.064.735682
ACACGAC45800.061.723333
ACGCTCT45550.061.2675368
CGCTCTT60250.047.1613049
CTTCCGA77700.08.50812210-14
TCTTCCG77550.08.3937210-14
TCCGATC85600.07.70597915-19
TTCCGAT87700.07.438799410-14
CCGATCT92500.07.17817315-19
CGATCTA26050.06.17739615-19
CGTAGAG25352.3323082E-65.8026951
TAACCCT585950.05.75238376
CTAACCC618700.05.7173385
CGGTGAG41505.0931703E-115.67126041
CCTAACC634300.05.553824
Produced by FastQC (version 0.11.5)