FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005485984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20152940
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1930912495.81293845959944No Hit
NAGTAGAG3417521.6957922764618958No Hit
AAGGAGAG591000.2932574602018366No Hit
AAGTCGAG589470.2924982657617201No Hit
AAGTGGAG369870.18353153435677375No Hit
AAGTAGCG364400.1808172901819784No Hit
AAGTTGAG355760.17653007452014446No Hit
AAGCAGAG325000.1612667928351893No Hit
ACGTAGAG314230.15592265942338934RNA PCR Primer, Index 17 (100% over 8bp)
AAGAAGAG297690.14771542018186926No Hit
AAGTAGAA228520.1133928846113768No Hit
AGGTAGAG228220.11324402295645201No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)