FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005485991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005485991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19777128
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA30650.083.837341
CGCCGAA31350.075.876521
TTACGCG11650.074.667489
TTTGCGC32700.073.6420751
GTGCGAC36950.071.529961
GCGCTAG34800.070.4639741
ACGACCG29900.069.979951
ATAACGC37700.068.938511
CGGTACG28800.068.828961
GCGCGTA33500.066.842851
CGAACGC37750.065.540971
TAGGCGC36050.065.37321
GACGCGT30950.064.522061
GTTCGCG42700.064.001438
TTACCCG38150.063.6991121
GGCGACT40150.063.268931
ACGCCGA37400.063.0134661
AACGCGT28400.062.0591353
TCACGCG25500.061.971578
GTTACGC28900.061.9548458
Produced by FastQC (version 0.11.5)