FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18763067
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT23900.086.740656
ACGACGC24250.086.3852165
CACGACG26650.078.06154
GACGCTC35500.059.0097857
TACACGA39700.052.9560132
CTACACG43150.050.0921971
ACGCTCT45050.046.8225488
ACACGAC51050.041.034993
CGCTCTT58250.036.7098739
CTTCCGA69200.07.018182310-14
TTCCGAT77250.06.774770710-14
TCTTCCG73100.06.703247510-14
CGTCGTT10850.0087930056.685768145
CGATGAG30051.2732926E-106.6283061
CGCCAAT16101.982539E-46.41483351
TCCGATC77300.06.395219315-19
CCGATCT84400.06.13205915-19
CGGGTTA24951.66684E-65.9134741
TAACCCT678150.05.9002156
CTAACCC712950.05.71387345
Produced by FastQC (version 0.11.5)