FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880574
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACG24900.085.883324
CGACGCT24400.085.56226
ACGACGC25700.081.516215
GACGCTC34700.059.7469677
TACACGA41300.051.814122
CTACACG45800.047.6194041
ACGCTCT43950.047.1667378
ACACGAC51300.042.109993
CGCTCTT59800.034.423329
CTTCCGA72800.06.79051710-14
TCTTCCG72850.06.56695810-14
TTCCGAT79350.06.22998910-14
TCCGATC80000.05.94420615-19
ACCGGGT22901.5329539E-55.81118151
TAACCCT719300.05.79478176
ACGGGAG42601.4551915E-115.72706031
CGATGGG35155.0240487E-95.6789211
CGTAGAG26253.9446077E-65.63284871
CTAACCC754750.05.62825545
CCGATCT88850.05.58057115-19
Produced by FastQC (version 0.11.5)