FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19108758
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG10750.079.566389
TGCGCGA28750.079.530951
CGCCGAA31300.079.455461
GTGCGAC34100.078.156181
ACGACCG26400.076.4871141
TTTGCGC31100.076.147141
CGGTACG29850.074.361851
CGTTGCG15050.072.245497
GCGCTAG31650.071.070971
GACGCGT31600.069.3039861
TCCGCGA27100.067.671728
AACGCGT25800.067.429483
ATAACGC36600.067.341121
GCCGGAT39550.066.260011
CCTTACG35950.065.6676561
TTACCCG33800.065.232351
ACGCCGA38300.064.933581
CCGCTAT36750.064.440161
ACCGCTT42900.063.1624371
CCGAACG20100.063.1099749
Produced by FastQC (version 0.11.5)