FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049430
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG12500.086.983979
TGCGCGA27850.080.315541
GTGCGAC30250.076.8622061
ACGACCG28550.076.800021
TTTGCGC30850.074.651811
CGGTACG26750.073.991131
GCGCTAG30200.073.5785451
CGCCGAA26700.073.578541
GCGCGTA27600.068.246761
GGCGACT36050.067.353451
CGTTGCG13500.067.117087
ACGCCGA32700.066.828221
CCGCTAT34450.066.423591
ATAACGC33250.066.386661
TAGGCGC34700.064.6727751
GTTACGC30100.063.5763248
TTACCCG31850.063.2983361
CGAACGC32900.063.0673181
CGCGGTA38150.062.8744541
GTACCGT39750.062.183518
Produced by FastQC (version 0.11.5)