FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005486023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18049430
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGC25350.099.2298365
CGACGCT24850.098.6009456
CACGACG26900.093.78244
GACGCTC36800.066.385087
TACACGA42000.060.1063652
CTACACG44850.057.0399931
ACGCTCT44350.055.729838
ACACGAC46400.054.525773
CGCTCTT59500.041.905379
CGATGGG32850.07.2024531
CTTCCGA83000.07.14368310-14
TCTTCCG78250.07.09563710-14
GTTCCGA25601.7844286E-96.7961365
TTCCGAT93400.06.550018310-14
TCCGATC91350.06.38029415-19
CCGATCT98450.06.12633615-19
CGTAAAG22951.5853897E-55.79903361
CGCTGAT25502.35864E-65.7990331
GTCGTCC16500.0022778375.71067679
AACGGAG34101.5023033E-85.6374771
Produced by FastQC (version 0.11.5)