FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005486026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005486026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20158012
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTAGAG1916162295.0571018610367No Hit
NAGTAGAG4021641.9950578459820345No Hit
AAGGAGAG693770.344165883024576No Hit
AAGTCGAG554220.2749378262102433No Hit
AAGAAGAG423360.21002071037560646No Hit
AAGTGGAG380490.18875373226288386No Hit
AAGTTGAG371650.18436837918342344No Hit
AAGCAGAG369120.18311329510072719No Hit
ACGTAGAG337110.1672337530109616RNA PCR Primer, Index 17 (100% over 8bp)
AAGTAGCG333090.1652395087372703No Hit
AGGTAGAG253310.12566219327580516No Hit
CAGTAGAG245550.12181260731464988No Hit
AAGTAGAA227370.1127938608231804No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)